Fig. 3
From: Pathogenesis of acephalic spermatozoa syndrome caused by PMFBP1 mutation
Immunofluorescence analysis of the spermatozoa from the control and from the patient. The acrosome, nucleus, and neck of the sperm were stained with CD46 (red), DAPI (blue) and PMFBP1 antibodies (green), respectively. The localization of PMFBP1 in sperm from control and patient samples was determined by immunofluorescence staining. The PMFBP1 protein is localized at the head–tail junction of spermatozoa in control samples but is not detected in patient spermatozoa. 40 times oil microscope observation, scale bar = 4 µm