Pathogenesis of acephalic spermatozoa syndrome caused by PMFBP1 mutation

Table 2 Primers used to construct minigenes

	F/R^a	Primer Sequence (5’ to 3’)
1^b	F	TTACAGGCGTTGGAGGAAAC
1^b	R	AAGTAGAGCTGTAGGTGGCC
2^c	F	GTTAACACGGACTCTGCAGG
2^c	R	CGATCTTTGCTGCCTCTGTC
3^d	F	AGCTCTACTTGTTAACACGGACTCTGCAGG
3^d	R	CCGTGTTAACAAGTAGAGCTGTAGGTGGCC
4^d	F	CTATAGGGAGACCCAAGCTTCACAGGGAGCAAGGCTCCA
4^d	R	ACGTCATATGGATAGGATCCCTCCAGCTCACTCTCCTGGT
5^e	F	ATCACGTGACCTCAGAGACAAAGAGCCTGCAGCAAA
5^e	R	TCTGAGGTCACGTGATTGAGCTTCCGGAAAAGACAAAG
6^f	F	AGACAAGACGTTGAAAGAG
6^f	R	CGTGATTGAGCTTCTCGAG

^aF represents the forward primer. R represents the reverse primer
^bIt was used to amplify exons 13–14 with complete intron 13 and partial intron 14
^cExons 15–16 with complete intron 15 and partial intron 14
^dThese sequences were used to connect two amplifications and construct wild-type minigenes
^eMutant minigenes were constructed
^fIt was used to determine the removal resulting from splice-site mutation

ISSN: 2051-4190